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Diagnostic Techniques of Microbiology, Virology, Mycology and Parasitology - mod.TECNICHE LABORATORY OF VIROLOGY
MARIA ELISABETTA MEGNA

Seat Medicina e Chirurgia
A.A. 2013-2014
Credits 2
Hours 20
Period 1^ semestre
Language ENG
U-gov code MT05-11-11 MT518

Prerequisites

CONCEPTS OF GENERAL BIOLOGY, CONCEPTS OF GENERAL CHEMISTRY, CONCEPTS OF IMMUNOLOGY.

Development of the course

THE COURSE INCLUDES 20 HOURS OF TEACHING.

LESSONS WILL TAKE PLACE IN ITALIAN WITH USE OF VIDEOPREIETTORE /POWER POINT PRESENTATIONS FOR PC.

Learning outcomes

THE COURSE OF STUDY IS A COMBINATION OF INSTRUCTION IN FUNDAMENTAL PRINCIPLES OF  VIROLOGY LABORATORY TECHNIQUES

Program

PREANALYTICAL PHASE:  FITNESS AND ACCEPTANCE OF BIOLOGICAL SAMPLE. BIOLOGICAL RISKS AND INDIVIDUAL SAFETY DEVICES .

MANUAL AND AUTOMATED TECHNIQUES OF  DIRECT DIAGNOSIS:

·         EXTRACTION OF VIRAL NUCLEIC ACID (DNA, RNA).

·         DETERMINATION OF VIRAL NUCLEIC ACIDS: PCR, NESTED-PCR, RT-PCR,REAL TIME.

·         GEL ELECTROPHORESIS ON THE PRODUCTS OF AMPLIFICATION .

·         RESEARCH OF VIRAL ANTIGENS.

MANUAL AND AUTOMATED TECHNIQUES OF  INDIRECT DIAGNOSIS:

• ENZYME  IMMUNOASSAY  TECHNIQUES: EIA,ELISA , MEIA, CHEMILUMINESCENCE, ELFA,                                                        IF, FDC, WESTERN BLOT.

POSTANALYTICAL PHASE AND CORRECT DISPOSAL OF BIOLOGICAL SAMPLES.

 

Development of the examination

ORAL TEST

Recommended reading

SLIDES / LECTURE NOTES PRODUCED BY PROFESSOR (AVAILABLE ON COMPUTER SUPPORT). THE REFERENCE TEXT WILL PRESS AS SOON AS POSSIBLE.

a)      TITLE

b)      AUTHOR

c)       PUBLISHER

Courses

• Tecniche di Laboratorio Biomedico