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Biochemistry and Clinical Pathology and Laboratory Organization - mod.TECNICHE LABORATORY OF CLINICAL PATHOLOGY
FABIOLA OLIVIERI

Seat Medicina e Chirurgia
A.A. 2013-2014
Credits 2
Hours 20
Period 1^ semestre
Language ENG
U-gov code MT05-11-11 MT276

Prerequisites
Basic knowledge of biochemistry, biology, histology , anatomy and physiology is recommended

Program

Extraction and storage of biological material for molecular analysis

Diagnostic techniques based on nucleic acids

Techniques for DNA amplification

Analysis of DNA mutations and polymorphisms (sequencing, pyrosequencing, restriction enzymes)
Study of gene expression by real-time PCR

Analysis of melting curves and identification of genetic polymorphisms by real-time PCR Applications of real-time PCR in clinical pathology

Analysis of DNA and RNA using arrays

Study of epigenetics: epigenetic states in human disease

Major applications of molecular diagnostic techniques in clinical pathology (diagnosis of genetic diseases, infectious diseases, study and diagnosis of cancer, etc..).

 

COURSE OBJECTIVES: knowledge of the main molecular methods and their applications in clinical pathology

 

Development of the examination

written

Recommended reading

a)      TITLE: MEDICINA DI LABORATORIO

b)      AUTHOR: LAPOSAT,

c)       PUBLISHER: PICCIN

 

 

Courses

• Tecniche di Laboratorio Biomedico